Alteration of urinary macromolecules by adsorption on surfaces, probably an important factor in urolithiasis

Baumann, J. M.; Affolter, B.; von Arx, U.; Noël, Marion (2013). Alteration of urinary macromolecules by adsorption on surfaces, probably an important factor in urolithiasis Urolithiasis, 41(6), pp. 467-474. Springer Verlag 10.1007/s00240-013-0604-5

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To get information on the consequences of adsorption of urinary macromolecules (UMs) on crystals, Ca phosphate (CaP) precipitation was performed in urine of 15 stone patients and 15 controls. In solutions of dissolved precipitates (DPU), Ca oxalate (CaOx) crystallization and aggregation (AGN) of latex beads were spectrophotometrically studied and compared to results obtained in urine and in UMs isolated by hemofilter dialysis (HD). Tests were repeated with a 20 lg/mL albumin solution (AS). Inhibition of CaOx growth and AGN was significantly reduced in DPU as well as in HD. Furthermore, DPU and HD showed an AGN of latex beads which in patients was more pronounced than in controls. Identical effects observed in DPU and HD can be explained by temporary high concentrations of UMs by adsorption on surfaces favoring self AGN. Temporary adsorption of AS on CaP produced polymers of a limited size, significantly reduced the inhibition of CaOx AGN and accelerated latex AGN. In DPU, AGN of latex beads occurred despite a surface potential of -37 mV, which within short distances produces areas of massive electrostatic repulsion and normally inhibits AGN. Such areas might be bridged by selfaggregated UMs. The retardation of CaOx AGN measured in DPU could be decisive whether during crystalluria crystals are washed out from the kidney by diuresis or are attached to kidney calcifications or pre-existing stones.

Item Type:

Journal Article (Original Article)

Division/Institute:

BFH Zentren > BFH Zentrum Holz - Ressource und Werkstoff
Department of Architecture, Wood and Civil Engineering
Department of Architecture, Wood and Civil Engineering > Bereich Forschung & Entwicklung, Dienstleistungen und Weiterbildung
Department of Architecture, Wood and Civil Engineering > Bereich Forschung & Entwicklung, Dienstleistungen und Weiterbildung > Institut für Werkstoffe und Holztechnologie

Name:

Baumann, J. M.; Affolter, B.; von Arx, U. and Noël, Marion

Subjects:

Q Science > Q Science (General)
Q Science > QD Chemistry
Q Science > QM Human anatomy
R Medicine > R Medicine (General)
R Medicine > RB Pathology
T Technology > TP Chemical technology

ISSN:

2194-7228

Publisher:

Springer Verlag

Language:

English

Submitter:

Christelle Ganne-Chédeville

Date Deposited:

21 Apr 2020 11:25

Last Modified:

21 Apr 2020 11:25

Publisher DOI:

10.1007/s00240-013-0604-5

Uncontrolled Keywords:

Adsorption Aggregation Calcium oxalate Self aggregation Urinary macromolecules

URI:

https://arbor.bfh.ch/id/eprint/11281

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