Co-hydrolysis of hydrothermal and dilute acid pretreated populus slurries to support development of a high-throughput pretreatment system

Studer, Michael Hans-Peter; Brethauer Studer, Simone; DeMartini, Jaclyn D; McKenzie, Heather L; Wyman, Charles E (2011). Co-hydrolysis of hydrothermal and dilute acid pretreated populus slurries to support development of a high-throughput pretreatment system Biotechnology for Biofuels, 4(1), p. 19. BioMed Central 10.1186/1754-6834-4-19

Preview

Text studer2011 Co-hydrolysis of hydrothermal and dilute acid pretreated populus slurries to support development of a high-throughput pretreatment system.pdf - Published Version Available under License Creative Commons: Attribution (CC-BY). Download (374kB) | Preview

Background The BioEnergy Science Center (BESC) developed a high-throughput screening method to rapidly identify low-recalcitrance biomass variants. Because the customary separation and analysis of liquid and solids between pretreatment and enzymatic hydrolysis used in conventional analyses is slow, labor-intensive and very difficult to automate, a streamlined approach we term 'co-hydrolysis' was developed. In this method, the solids and liquid in the pretreated biomass slurry are not separated, but instead hydrolysis is performed by adding enzymes to the whole pretreated slurry. The effects of pretreatment method, severity and solids loading on co-hydrolysis performance were investigated. Results For hydrothermal pretreatment at solids concentrations of 0.5 to 2%, high enzyme protein loadings of about 100 mg/g of substrate (glucan plus xylan) in the original poplar wood achieved glucose and xylose yields for co-hydrolysis that were comparable with those for washed solids. In addition, although poplar wood sugar yields from co-hydrolysis at 2% solids concentrations fell short of those from hydrolysis of washed solids after dilute sulfuric acid pretreatment even at high enzyme loadings, pretreatment at 0.5% solids concentrations resulted in similar yields for all but the lowest enzyme loading. Conclusions Overall, the influence of severity on susceptibility of pretreated substrates to enzymatic hydrolysis was clearly discernable, showing co-hydrolysis to be a viable approach for identifying plant-pretreatment-enzyme combinations with substantial advantages for sugar production.

Item Type:	Journal Article (Original Article)
Division/Institute:	School of Agricultural, Forest and Food Sciences HAFL > Resource-efficient agricultural production systems School of Agricultural, Forest and Food Sciences HAFL > Agriculture
Name:	Studer, Michael Hans-Peter0000-0003-1083-0967; Brethauer Studer, Simone0000-0001-7755-7593; DeMartini, Jaclyn D; McKenzie, Heather L and Wyman, Charles E
Subjects:	Q Science > Q Science (General)
ISSN:	1754-6834
Publisher:	BioMed Central
Language:	English
Submitter:	Michael Hans-Peter Studer
Date Deposited:	18 Feb 2020 08:09
Last Modified:	18 Dec 2020 13:29
Publisher DOI:	10.1186/1754-6834-4-19
Uncontrolled Keywords:	Xylose, Solid Concentration, Sugar Yield, Glucose Yield, Enzyme Loading
ARBOR DOI:	10.24451/arbor.10129
URI:	https://arbor.bfh.ch/id/eprint/10129