Heydenreich, Juliane; Otto, Christoph; Mayer, Frank; Carlsohn, Anja (2014). Reliability of a Fully Automated Interpretation of Journal of Nutrition and Metabolism, 2014(3), p. 478324. Hindawi 10.1155/2014/478324
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Heydenreich_2014_Reliability of a Fully Automated Interpretation of γ-H2AX Foci in Lymphocytes of Moderately Trained Subjects under Resting Conditions.pdf - Published Version Available under License Creative Commons: Attribution (CC-BY). Download (529kB) | Preview |
Background: Analysis of γ-H2AX foci is a promising approach to evaluate exercise-induced DNA damage. However, baseline levels and day-to-day variability of γ-H2AX foci have not been investigated in healthy subjects at rest. Methods: Blood was taken from eight moderately trained healthy males (29 ± 3 yrs, 1.84 ± 0.03 m, and 85 ± 6 kg) at two separate days (M1/M2) after 24-hour exercise cessation. Number of γ-H2AX foci per 100 lymphocytes (N), number of foci per affected lymphocyte (NAL), percentage of affected lymphocytes (PAL), and diameter (D) of γ-H2AX foci were analyzed (mean ± SD). Differences between M1 and M2 were analyzed using paired t-tests (α = 0.05). Day-to-day variability was evaluated by calculating the coefficients of variation (CV%), bias, and limits of agreement (LoA). Results: There were no statistically significant differences between M1 (N: 7.6 ± 4.4, NAL: 1.2 ± 0.2, PAL: 5.9 ± 2.6%, and D: 0.63 ± 0.07) and M2 (N: 8.4 ± 4.6, NAL: 1.3 ± 0.1, PAL: 6.9 ± 4.2%, and D: 0.66 ± 0.06). CV was calculated to be 98.5% (N), 88.9% (PAL), 11.3% (NAL), and 8.0% (D). Bias (LoA) was 0.75 (-15.2/13.7), -0.02 (-0.36/0.33), -1.0 (-11.9/9.9), and -0.04 (-0.16/0.09), respectively. Conclusions: Background level in healthy subjects is approximately 0.07 to 0.09 γ-H2AX foci/cell. NAL and D are reliable measures.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
Swiss Federal Institute of Sports Magglingen SFISM > EHSM - Leistungssport |
Name: |
Heydenreich, Juliane; Otto, Christoph; Mayer, Frank and Carlsohn, Anja |
ISSN: |
2090-0724 (Print) 2090-0732 (Online) |
Publisher: |
Hindawi |
Language: |
English |
Submitter: |
Service Account |
Date Deposited: |
22 Mar 2021 10:01 |
Last Modified: |
12 Oct 2021 02:18 |
Publisher DOI: |
10.1155/2014/478324 |
Related URLs: |
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PubMed ID: |
25147735 |
ARBOR DOI: |
10.24451/arbor.11146 |
URI: |
https://arbor.bfh.ch/id/eprint/11146 |
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