La Marca, RobertoRobertoLa MarcaBoesch, MariaMariaBoeschSefidan, SandraSandraSefidanEhlert, UlrikeUlrikeEhlertAnnen, HubertHubertAnnenWyss, ThomasThomasWyssSteptoe, AndrewAndrewSteptoe2024-11-192024-11-1920140306-4530 (Print) 1873-3360 (Online)10.24451/arbor.11037https://doi.org/10.24451/arbor.1103710.1016/j.psyneuen.2014.02.003https://arbor.bfh.ch/handle/arbor/32236Introduction: A group version of the Trier Social Stress Test (TSST-G) was introduced as astandardized, economic and efficient tool to induce a psychobiological stress response simulta-neously in a group of subjects. The aim of the present study was to examine the efficacy of theTSST-G to repeatedly induce an affective and autonomic stress response while comparing twoalternative protocols for the second examination.Methods and materials: Healthy young male recruits participated twice in the TSST-G 10 weeksapart. In the first examination, the TSST-G consisted of a combination of mental arithmetic and afake job interview (TSST-G-1st; n = 294). For the second examination, mental arithmetic wascombined with either (a) a defensive speech in response to a false shoplifting accusation (TSST-G-2nd-defence; n = 105), or (b) a speech on a more neutral topic selected by the investigators (TSST-G-2nd-presentation; n = 100). Affect ratings and salivary alpha-amylase (sAA) were determinedimmediately before and after the stress test, while heart rate (HR) and heart rate variability(HRV) were measured continuously.enTrier Social Stress Test for Groups Repeated stressprovocation Autonomic nervoussystem Salivary alpha-amylase Heart rate Heart rate variabilityarticle