Membrane Shape at the Edge of the Dynamin Helix Sets Location and Duration of the Fission Reaction

Morlot, Sandrine; Galli, Valentina; Klein, Marius Jan; Chiaruttini, Nicolas; Manzi, John; Humbert, Frederic; Dinis, Luis; Lenz, Martin; Cappello, Giovanni; Roux, Aurelien (2012). Membrane Shape at the Edge of the Dynamin Helix Sets Location and Duration of the Fission Reaction Cell, 151(3), pp. 619-629. Cell Press 10.1016/j.cell.2012.09.017

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The GTPase dynamin polymerizes into a helical coat that constricts membrane necks of endocytic pits to promote their fission. However, the dynamin mechanism is still debated because constriction is necessary but not sufficient for fission. Here, we show that fission occurs at the interface between the dynamin coat and the uncoated membrane. At this location, the considerable change in membrane curvature increases the local membrane elastic energy, reducing the energy barrier for fission. Fission kinetics depends on tension, bending rigidity, and the dynamin constriction torque. Indeed, we experimentally find that the fission rate depends on membrane tension in vitro and during endocytosis in vivo. By estimating the energy barrier from the increased elastic energy at the edge of dynamin and measuring the dynamin torque, we show that the mechanical energy spent on dynamin constriction can reduce the energy barrier for fission sufficiently to promote spontaneous fission.

Item Type:	Journal Article (Original Article)
Division/Institute:	Business School > Institute for Applied Data Science & Finance Business School
Name:	Morlot, Sandrine; Galli, Valentina; Klein, Marius Jan; Chiaruttini, Nicolas; Manzi, John; Humbert, Frederic; Dinis, Luis; Lenz, Martin; Cappello, Giovanni and Roux, Aurelien
ISSN:	0092-8674
Publisher:	Cell Press
Language:	English
Submitter:	Marius Jan Klein
Date Deposited:	26 Aug 2024 15:26
Last Modified:	26 Aug 2024 15:26
Publisher DOI:	10.1016/j.cell.2012.09.017
Additional Information:	Publisher: Elsevier
ARBOR DOI:	10.24451/arbor.22272
URI:	https://arbor.bfh.ch/id/eprint/22272