Development of a new in vivo protocol through soil inoculation to investigate sugar beet resistance towards Ditylenchus dipsaci penetration

Storelli, Alan; Keiser, Andreas; Kiewnick, Sebastian; Daub, Matthias; Mahlein, Anne-Katrin; Beyer, Werner; Schumann, Mario (2021). Development of a new in vivo protocol through soil inoculation to investigate sugar beet resistance towards Ditylenchus dipsaci penetration Nematology, 23(6), pp. 685-694. Brill 10.1163/15685411-bja10069

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The stem nematode,Ditylenchus dipsaci, causes severe damage in sugar beet. To date, nematode inoculation throughthe leaf axil has been used as the standard method to investigateD. dipsaciinteraction with sugar beet underin vivoconditions. Toget as close as possible to field conditions, we established a new screening mechanism to perform soil inoculation. The most suitableinoculation time point, inoculum level and positioning on sugar beet, as well as rearing process on carrots, were determined. At a15:8°C day:night temperature regime, penetration rates ofD. dipsaciwere at maximum following soil inoculation at plant emergence.Up to 115 nematodes penetrated sugar beet seedlings 22 days post-planting with an inoculum level of 1000 nematodes into the soil atplant emergence.Ditylenchus dipsacipenetration rate was higher in plants with soil inoculation than with inoculation on to the leaf axil.High soil moisture increased nematode migration into seedlings whenD. dipsaciinoculation was carried out in four holes 1 cm fromthe plant base. Rearing the nematodes for 35 days at 20°C on carrot discs resulted in an infective inoculum containing up to 50% eggs.We recommend a soil inoculation of 1000 freshly extracted nematodes per pot at plant emergence. The nematode suspension has to bepreviously reared for 35 days on carrot discs to obtain activeD. dipsaciinoculum. This system will allow for the selection of suitablesugar beet genotypes that suppress nematode penetration, in support of breeding for resistance againstD. dipsaci.

Item Type:	Journal Article (Original Article)
Division/Institute:	School of Agricultural, Forest and Food Sciences HAFL School of Agricultural, Forest and Food Sciences HAFL > Agriculture School of Agricultural, Forest and Food Sciences HAFL > Agriculture > Plant Production and Biodiversity
Name:	Storelli, Alan0000-0001-5107-0023; Keiser, Andreas; Kiewnick, Sebastian; Daub, Matthias; Mahlein, Anne-Katrin; Beyer, Werner and Schumann, Mario
Subjects:	S Agriculture > SB Plant culture
ISSN:	1568-5411
Publisher:	Brill
Funders:	[UNSPECIFIED] KWS SAAT SE & Co. KGaA ; [UNSPECIFIED] Schweizer Zucker ; [UNSPECIFIED] Berner Fachhochscule
Projects:	[UNSPECIFIED] Auf der Suche nach resistenten Zuckerrüben gegen Stängelnematode
Language:	English
Submitter:	Alan Storelli
Date Deposited:	04 Feb 2021 13:28
Last Modified:	24 Jan 2022 11:30
Publisher DOI:	10.1163/15685411-bja10069
Uncontrolled Keywords:	Beta vulgaris, carrot disc rearing, inoculum, plant breeding, seedling test system, stem nematode
ARBOR DOI:	10.24451/arbor.14115
URI:	https://arbor.bfh.ch/id/eprint/14115