Small-scale and automatable high-throughput compositional analysis of biomass

DeMartini, Jaclyn D.; Studer, Michael Hans-Peter; Wyman, Charles E. (2010). Small-scale and automatable high-throughput compositional analysis of biomass Biotechnology and Bioengineering, 108(2), pp. 306-312. John Wiley & Sons 10.1002/bit.22937

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Conventional wet chemistry methods to determine biomass composition are labor- and time-intensive and require larger amounts of biomass (300 mg) than is often available. To overcome these limitations and to support a high-throughput pretreatment and hydrolysis (HTPH) screening system, this article reports on the development of a downscaled biomass compositional analysis that is based on conventional wet chemistry techniques but is scaled down by a factor of 100 to use significantly less material. The procedure is performed in readily available high-performance liquid chromatography vials and can be automated to reduce operator input and increase throughput. Comparison of the compositional analyses of three biomasses determined by the downscaled approach to those obtained by conventional methods showed that the downscaled method measured statistically identical carbohydrate compositions as standard procedures and also can provide reasonable estimates of lignin and ash contents. These results demonstrate the validity of the downscaled procedure for measuring biomass composition to enable the calculation of sugar yields and determination of trends in sugar release behavior in HTPH screening studies.

Item Type:	Journal Article (Original Article)
Division/Institute:	School of Agricultural, Forest and Food Sciences HAFL > Resource-efficient agricultural production systems School of Agricultural, Forest and Food Sciences HAFL > Agriculture
Name:	DeMartini, Jaclyn D.; Studer, Michael Hans-Peter0000-0003-1083-0967 and Wyman, Charles E.
Subjects:	Q Science > Q Science (General)
ISSN:	00063592
Publisher:	John Wiley & Sons
Language:	English
Submitter:	Michael Hans-Peter Studer
Date Deposited:	19 Feb 2020 16:01
Last Modified:	18 Dec 2020 13:29
Publisher DOI:	10.1002/bit.22937
Additional Information:	Gemäss Verlagswebsite erschien der Artikel am: February 2011 in der Zeitschrift Biotechnology and Bioengineering
ARBOR DOI:	10.24451/arbor.10128
URI:	https://arbor.bfh.ch/id/eprint/10128