The role of TRPV6 in breast carcinogenesis

Kopf, Katrin Annika; Hediger, M. A.; Landowski, C. P. (2008). The role of TRPV6 in breast carcinogenesis Molecular Cancer Therapeutics, 7(2), pp. 271-279. American Association for Cancer Research 10.1158/1535-7163.MCT-07-0478

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TRPV6 is an endothelial calcium entry channel that is strongly expressed in breast adenocarcinoma tissue. In this study, we further confirmed this observation by analysis of breast cancer tissues, which indicated that TRPV6 mRNA expression was up-regulated between 2-fold and 15-fold compared with the average in normal breast tissue. Whereas TRPV6 is expressed in the cancer tissue, its role as a calcium channel in breast carcinogenesis is poorly understood. Therefore, we investigated how TRPV6 affects the viability, apoptosis, and calcium transport in the breast cancer cell line T47D. Hormones can also affect the tumor development; hence, we determined the effects of estradiol, progesterone, and 1,25-vitamin D on TRPV6 transcription. Interestingly, the estrogen receptor antagonist tamoxifen reduced expression of TRPV6 and is able to inhibit its calcium transport activity (IC(50), 7.5 micromol/L). The in vitro model showed that TRPV6 can be regulated by estrogen, progesterone, tamoxifen, and 1,25-vitamin D and has a large influence on breast cancer cell proliferation. Moreover, the effect of tamoxifen on cell viability was enhanced when TRPV6 expression was silenced with small interfering RNA. TRPV6 may be a novel target for the development of calcium channel inhibitors to treat breast adenocarcinoma expressing TRPV6.

Item Type:	Journal Article (Original Article)
Division/Institute:	School of Agricultural, Forest and Food Sciences HAFL > Consumer-focused Food Production
Name:	Kopf, Katrin Annika0000-0002-7443-7489; Hediger, M. A. and Landowski, C. P.
Subjects:	R Medicine > R Medicine (General)
ISSN:	1535-7163
Publisher:	American Association for Cancer Research
Language:	English
Submitter:	Katrin Annika Kopf
Date Deposited:	24 Feb 2020 07:23
Last Modified:	18 Dec 2020 13:29
Publisher DOI:	10.1158/1535-7163.MCT-07-0478
ARBOR DOI:	10.24451/arbor.9252
URI:	https://arbor.bfh.ch/id/eprint/9252