Lignin repolymerisation in spruce autohydrolysis pretreatment increases cellulase deactivation

Pielhop, Thomas; Larrazábal, Gastón O.; Studer, Michael Hans-Peter; Brethauer Studer, Simone; Seidel, Christoph-M.; Rudolf von Rohr, Philipp (2015). Lignin repolymerisation in spruce autohydrolysis pretreatment increases cellulase deactivation Green Chemistry, 17(6), pp. 3521-3532. Royal Society of Chemistry 10.1039/C4GC02381A

[img]
Preview
Text
c4gc02381a.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (2MB) | Preview

This study presents a modified autohydrolysis pretreatment which helps to overcome the recalcitrance of softwood for enzymatic hydrolysis of its cellulose. Autohydrolysis pretreatments of spruce wood were performed with 2-naphthol, which prevents lignin repolymerisation reactions, thereby increasing the enzymatic digestibility of cellulose by up to 64%. The negative influence of repolymerised lignin structures on enzymatic hydrolysis was confirmed by the addition of resorcinol in autohydrolysis, which is known to promote repolymerisation reactions and decreased the biomass digestibility. Several analyses were performed to study the underlying mechanism of this effect on hydrolysis, indicating that cellulolytic enzymes are adsorbed and deactivated especially by repolymerised lignin structures, which accounts for the high differences in biomass digestibility. It was shown that lignin repolymerisation significantly increases its specific surface area through modification of the lignin nanostructure, which is supposed to increase the unproductive binding of enzymes.

Item Type:

Journal Article (Original Article)

Division/Institute:

School of Agricultural, Forest and Food Sciences > Abteilung Agronomie

Name:

Pielhop, Thomas; Larrazábal, Gastón O.; Studer, Michael Hans-Peter; Brethauer Studer, Simone; Seidel, Christoph-M. and Rudolf von Rohr, Philipp

Subjects:

S Agriculture > S Agriculture (General)
T Technology > TP Chemical technology

ISSN:

1463-9262

Publisher:

Royal Society of Chemistry

Language:

English

Submitter:

Simon Lutz

Date Deposited:

18 Feb 2020 08:07

Last Modified:

18 Feb 2020 08:07

Publisher DOI:

10.1039/C4GC02381A

ARBOR DOI:

10.24451/arbor.8355

URI:

https://arbor.bfh.ch/id/eprint/8355

Actions (login required)

View Item View Item
Provide Feedback