Pielhop, Thomas; Larrazábal, Gastón O.; Studer, Michael Hans-Peter; Brethauer Studer, Simone; Seidel, Christoph-M.; Rudolf von Rohr, Philipp (2015). Lignin repolymerisation in spruce autohydrolysis pretreatment increases cellulase deactivation Green Chemistry, 17(6), pp. 3521-3532. Royal Society of Chemistry 10.1039/C4GC02381A
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This study presents a modified autohydrolysis pretreatment which helps to overcome the recalcitrance of softwood for enzymatic hydrolysis of its cellulose. Autohydrolysis pretreatments of spruce wood were performed with 2-naphthol, which prevents lignin repolymerisation reactions, thereby increasing the enzymatic digestibility of cellulose by up to 64%. The negative influence of repolymerised lignin structures on enzymatic hydrolysis was confirmed by the addition of resorcinol in autohydrolysis, which is known to promote repolymerisation reactions and decreased the biomass digestibility. Several analyses were performed to study the underlying mechanism of this effect on hydrolysis, indicating that cellulolytic enzymes are adsorbed and deactivated especially by repolymerised lignin structures, which accounts for the high differences in biomass digestibility. It was shown that lignin repolymerisation significantly increases its specific surface area through modification of the lignin nanostructure, which is supposed to increase the unproductive binding of enzymes.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
School of Agricultural, Forest and Food Sciences HAFL > Resource-efficient agricultural production systems School of Agricultural, Forest and Food Sciences HAFL > Agriculture |
Name: |
Pielhop, Thomas; Larrazábal, Gastón O.; Studer, Michael Hans-Peter  0000-0003-1083-0967; Brethauer Studer, Simone 0000-0001-7755-7593; Seidel, Christoph-M. and Rudolf von Rohr, Philipp |
Subjects: |
S Agriculture > S Agriculture (General) T Technology > TP Chemical technology |
ISSN: |
1463-9262 |
Publisher: |
Royal Society of Chemistry |
Language: |
English |
Submitter: |
Simon Lutz |
Date Deposited: |
18 Feb 2020 08:07 |
Last Modified: |
18 Dec 2020 13:29 |
Publisher DOI: |
10.1039/C4GC02381A |
ARBOR DOI: |
10.24451/arbor.8355 |
URI: |
https://arbor.bfh.ch/id/eprint/8355 |
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