Frazer, Sarah; Prados, Julien; Niquille, Mathieu; Cadilhac, Christelle; Markopoulos, Foivos; Gomez Teijeiro, Lucia; Tomasello, Ugo; Telley, Ludovic; Holtmaat, Anthony; Jabaudon, Denis; Dayer, Alexandre (2017). Transcriptomic and anatomic parcellation of 5-HT3AR expressing cortical interneuron subtypes revealed by single-cell RNA sequencing Nature Communications, 8(1), pp. 1-12. 10.1038/ncomms14219
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Cortical GABAergic interneurons constitute a highly diverse population of inhibitory neurons that are key regulators of cortical microcircuit function. An important and heterogeneous group of cortical interneurons specifically expresses the serotonin receptor 3A (5-HT3AR) but how this diversity emerges during development is poorly understood. Here we use single-cell transcriptomics to identify gene expression patterns operating in Htr3a-GFP+ interneurons during early steps of cortical circuit assembly. We identify three main molecular types of Htr3a-GFP+ interneurons, each displaying distinct developmental dynamics of gene expression. The transcription factor Meis2 is specifically enriched in a type of Htr3a-GFP+ interneurons largely confined to the cortical white matter. These MEIS2-expressing interneurons appear to originate from a restricted region located at the embryonic pallial–subpallial boundary. Overall, this study identifies MEIS2 as a subclass-specific marker for 5-HT3AR-containing interstitial interneurons and demonstrates that the transcriptional and anatomical parcellation of cortical interneurons is developmentally coupled.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
Business School > Institute for Applied Data Science & Finance Business School |
Name: |
Frazer, Sarah; Prados, Julien; Niquille, Mathieu; Cadilhac, Christelle; Markopoulos, Foivos; Gomez Teijeiro, Lucia; Tomasello, Ugo; Telley, Ludovic; Holtmaat, Anthony; Jabaudon, Denis and Dayer, Alexandre |
Subjects: |
Q Science > Q Science (General) Q Science > QP Physiology R Medicine > R Medicine (General) |
ISSN: |
2041-1723 |
Language: |
English |
Submitter: |
Lucia Gomez Teijeiro |
Date Deposited: |
21 Aug 2024 11:30 |
Last Modified: |
21 Aug 2024 11:30 |
Publisher DOI: |
10.1038/ncomms14219 |
ARBOR DOI: |
10.24451/arbor.22051 |
URI: |
https://arbor.bfh.ch/id/eprint/22051 |
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