Three-Differential White Blood Cell Count in Capillary Driven Image Flow Cytometry

Stark, Matt; Vaerst, Clara; Hörr, Johannes; Bessire, Cédric (23 April 2022). Three-Differential White Blood Cell Count in Capillary Driven Image Flow Cytometry In: ICBET '22: Proceedings of the 12th International Conference on Biomedical Engineering and Technology (pp. 57-60). New York: ACM - Association for Computing Machinery 10.1145/3535694.3535705

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White blood cells (WBCs) are a heterogeneous group of nucleated cells that play an important role in human health and immunity. Devices to measure total the WBC count and relative sub-type counts in a blood sample are widely used in diagnostic medicine to gain insight into the health of patients. We demonstrate an image-based flow cytometry method to determine the three-differential counts of lymphocytes, monocytes, and granulocytes, along with the total WBC count using acridine orange infused whole blood flowing through a microfluidic channel. A capillary driven microfluidic device is used, which has the advantage over conventional flow cytometry systems in terms of its small size, mechanical simplicity, and robustness making it suitable for use in portable point-of-care devices. Detection of the three classes of WBCs is done using a standard lab fluorescence microscope and imaging sensor.

Item Type:	Conference or Workshop Item (Paper)
Division/Institute:	School of Engineering and Computer Science > Institute for Human Centered Engineering (HUCE) School of Engineering and Computer Science > Institute for Human Centered Engineering (HUCE) > HUCE / Laboratory for Microelectronics and Medical Devices School of Engineering and Computer Science
Name:	Stark, Matt; Vaerst, Clara; Hörr, Johannes and Bessire, Cédric
Subjects:	T Technology > T Technology (General) T Technology > TK Electrical engineering. Electronics Nuclear engineering
Publisher:	ACM - Association for Computing Machinery
Language:	English
Submitter:	Cédric Bessire
Date Deposited:	22 Nov 2022 15:30
Last Modified:	22 Nov 2022 15:30
Publisher DOI:	10.1145/3535694.3535705
Related URLs:	Organisation
ARBOR DOI:	10.24451/arbor.18027
URI:	https://arbor.bfh.ch/id/eprint/18027