Optimization of bulked AFLP analysis and its application for exploring diversity of natural and cultivated populations of red clover

Herrmann, Doris; Boller, Beat; Widmer, Franco; Kölliker, Roland (2005). Optimization of bulked AFLP analysis and its application for exploring diversity of natural and cultivated populations of red clover Genome, 48(3), pp. 474-486. Canadian Science Publishing 10.1139/G05-011

Text Herrmann_Genome_2005.pdf - Published Version Restricted to registered users only Available under License Publisher holds Copyright. Download (279kB) | Request a copy

Landraces and wild populations of red clover (Trifolium pratense L.) may represent a significant yet poorly characterized genetic resource of temperate grasslands. A bulking strategy with amplified fragment length polymorphism (AFLP) markers was optimized to characterize 120 red clover populations in 6 different groups: Swiss wild clover populations, Mattenklee landraces, Mattenklee cultivars, field clover cultivars, Dutch wild clover populations, and Dutch landraces. Analysis of 2 bulked samples/population consisting of 20 plants each with12 AFLP primer combinations was found optimal for determining genetic diversity and relationships within and among red clover populations and groups. Swiss wild clover populations were clearly separated from all other red clover groups and variability within and among populations was shown to be particularly high in wild clover populations and Mattenklee landraces, emphasising their value as genetic resources for improvement of red clover cultivars, as well as for conservation and restoration of biodiversity. This study shows that the ancestry of red clover landraces is primarily found in introduced cultivars rather than in natural wild clover populations. In addition, the methodological considerations presented here may help improve diversity analyses using bulked samples.

Item Type:	Journal Article (Original Article)
Division/Institute:	School of Agricultural, Forest and Food Sciences HAFL
Name:	Herrmann, Doris0000-0002-1776-9479; Boller, Beat; Widmer, Franco and Kölliker, Roland
Subjects:	S Agriculture > SB Plant culture
ISSN:	0831-2796
Publisher:	Canadian Science Publishing
Language:	English
Submitter:	Doris Herrmann
Date Deposited:	04 Aug 2021 09:17
Last Modified:	30 Sep 2021 02:18
Publisher DOI:	10.1139/G05-011
Uncontrolled Keywords:	AFLP, bulked samples, Trifolium pratense L., cultivars, landraces, wild populations
ARBOR DOI:	10.24451/arbor.15240
URI:	https://arbor.bfh.ch/id/eprint/15240