Sequential SEM imaging of microbial calcite precipitation consolidation treatment

Scherrer, Nadim; Kocsis, Matthias; Dariz, Petra; Gervais, Claire (2021). Sequential SEM imaging of microbial calcite precipitation consolidation treatment The European Physical Journal Plus, 136(5), pp. 1-10. Springer 10.1140/epjp/s13360-021-01497-7

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Cultural heritage built from limestone is prone to deterioration by chemical weathering, a natural process, that is enhanced by pollution. There are many historic monuments built from calcareous rocks that suffer from deterioration, and thus there have been a number of approaches over the last few decades to consolidate these types of rocks and surfaces. Using natural biological processes by fostering the activity of calcite-producing bacteria, also referred to as biomineralization, is one strategy that has also been commercialized. The base of proving the effectiveness of any surface treatment is the observation of the surface at sequential stages before and after treatment, as well as after exposure to weathering. Due to the heterogeneity of natural materials and processes, our aim was to observe identical test areas at the micron scale throughout the observation period. In order to achieve this on a tungsten SEM, we employed a beam deceleration accessory that allowed low kV imaging on non-conductive surfaces at a sufficiently high image resolution with a modified sample holder accommodating drill cores of 25 mm diameter and up to 15 mm height. The presented method is capable of producing time-sequenced images on the same test area on natural rock surface samples without manipulation for imaging purposes. This offers interesting perspectives for effective documentation of such processes in various fields.

Item Type:

Journal Article (Original Article)

Division/Institute:

Bern Academy of the Arts
Bern Academy of the Arts > Institute Materiality in Art and Culture
Bern Academy of the Arts > Institute Materiality in Art and Culture > Material analyses

Name:

Scherrer, Nadim0000-0002-6576-885X;
Kocsis, Matthias;
Dariz, Petra and
Gervais, Claire0000-0003-2433-5537

Subjects:

N Fine Arts > NA Architecture
Q Science > QC Physics
Q Science > QE Geology
Q Science > QR Microbiology

ISSN:

2190-5444

Publisher:

Springer

Language:

English

Submitter:

Nadim Scherrer

Date Deposited:

25 May 2021 15:31

Last Modified:

25 May 2021 15:31

Publisher DOI:

10.1140/epjp/s13360-021-01497-7

Related URLs:

ARBOR DOI:

10.24451/arbor.14860

URI:

https://arbor.bfh.ch/id/eprint/14860

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