Steam Explosion Pretreatment of Beechwood. Part 2: Quantification of Cellulase Inhibitors and Their Effect on Avicel Hydrolysis

Brethauer Studer, Simone; Antczak, Andrzej; Balan, Robert; Zielenkiewicz, Tomasz; Studer, Michael Hans-Peter (2020). Steam Explosion Pretreatment of Beechwood. Part 2: Quantification of Cellulase Inhibitors and Their Effect on Avicel Hydrolysis Energies, 13(14), p. 3638. MDPI 10.3390/en13143638

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Steam explosion is a well-known process to pretreat lignocellulosic biomass in order to enhance sugar yields in enzymatic hydrolysis, but pretreatment conditions have to be optimized individually for each material. In this study, we investigated how the results of a pretreatment optimization procedure are influenced by the chosen reaction conditions in the enzymatic hydrolysis. Beechwood was pretreated by steam explosion and the resulting biomass was subjected to enzymatic hydrolysis at glucan loadings of 1% and 5% employing either washed solids or the whole pretreatment slurry. For enzymatic hydrolysis in both reaction modes at a glucan loading of 1%, the glucose yields markedly increased with increasing severity and with increasing pretreatment temperature at identical severities and maximal values were reached at a pretreatment temperature of 230 °C. However, the optimal severity was 5.0 for washed solids enzymatic hydrolysis, but only 4.75 for whole slurry enzymatic hydrolysis. When the glucan loading was increased to 5%, glucose yields hardly increased for pretreatment temperatures between 210 and 230 °C at a given severity, and a pretreatment temperature of 220 °C was sufficient under these conditions. Consequently, it is important to precisely choose the desired conditions of the enzymatic hydrolysis reaction, when aiming to optimize the pretreatment conditions for a certain biomass.

Item Type:	Journal Article (Original Article)
Division/Institute:	School of Agricultural, Forest and Food Sciences HAFL School of Agricultural, Forest and Food Sciences HAFL > Agriculture School of Agricultural, Forest and Food Sciences HAFL > Agriculture > Sustainability and Circular Economy
Name:	Brethauer Studer, Simone0000-0001-7755-7593; Antczak, Andrzej; Balan, Robert; Zielenkiewicz, Tomasz and Studer, Michael Hans-Peter0000-0003-1083-0967
Subjects:	Q Science > Q Science (General)
ISSN:	1996-1073
Publisher:	MDPI
Language:	English
Submitter:	Michael Hans-Peter Studer
Date Deposited:	08 Jan 2021 13:09
Last Modified:	08 Jan 2021 13:09
Publisher DOI:	10.3390/en13143638
Uncontrolled Keywords:	steam explosion pretreatment; enzymatic hydrolysis; inhibition; HMF; phenolics; furfural
ARBOR DOI:	10.24451/arbor.14121
URI:	https://arbor.bfh.ch/id/eprint/14121