Engineering of a high-throughput screening system to identify cellulosic biomass, pretreatments, and enzyme formulations that enhance sugar release

Studer, Michael Hans-Peter; DeMartini, Jaclyn D.; Brethauer Studer, Simone; McKenzie, Heather L.; Wyman, Charles E. (2010). Engineering of a high-throughput screening system to identify cellulosic biomass, pretreatments, and enzyme formulations that enhance sugar release Biotechnology and Bioengineering, 105(2), pp. 231-238. John Wiley & Sons 10.1002/bit.22527

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The recalcitrance of cellulosic biomass, the only abundant, sustainable feedstock for making liquid fuels, is a primary obstacle to low cost biological processing, and development of more easily converted plants and more effective enzymes would be of great benefit. Because no single parameter describes recalcitrance, superior variants can only be identified by measuring sugar release from plants subjected to pretreatment and enzymatic hydrolysis. However, genetic modifications of plants coupled with molecular engineering of deconstruction proteins and definition of pretreatment conditions create a very large sample set, and previous methods for biomass pretreatment at elevated temperatures and pressures prevented use of a fully integrated high-throughput (HTP) screening pipeline. Herein, we report on the engineering of a novel HTP pretreatment system employing a 96 well-plate format that withstands extreme pretreatment conditions for rapid screening of biomass-enzyme-pretreatment combinations. This includes the development of new approaches to steam heating and water quenching the system that result in much faster heat up and cool down than previously possible and show consistent temperature histories across the multiwell plate. Coupled pretreatment and enzymatic hydrolysis performance of the well plate pretreatment system is shown to be consistent among the many wells in the device and also with performance of conventional tubular reactors.

Item Type:	Journal Article (Original Article)
Division/Institute:	School of Agricultural, Forest and Food Sciences HAFL > Resource-efficient agricultural production systems School of Agricultural, Forest and Food Sciences HAFL > Agriculture
Name:	Studer, Michael Hans-Peter0000-0003-1083-0967; DeMartini, Jaclyn D.; Brethauer Studer, Simone0000-0001-7755-7593; McKenzie, Heather L. and Wyman, Charles E.
Subjects:	Q Science > Q Science (General)
ISSN:	00063592
Publisher:	John Wiley & Sons
Language:	English
Submitter:	Michael Hans-Peter Studer
Date Deposited:	19 Feb 2020 16:00
Last Modified:	18 Dec 2020 13:29
Publisher DOI:	10.1002/bit.22527
ARBOR DOI:	10.24451/arbor.10130
URI:	https://arbor.bfh.ch/id/eprint/10130