The effect of bovine serum albumin on batch and continuous enzymatic cellulose hydrolysis mixed by stirring or shaking

Brethauer Studer, Simone; Studer, Michael Hans-Peter; Yang, Bin; Wyman, Charles E. (2011). The effect of bovine serum albumin on batch and continuous enzymatic cellulose hydrolysis mixed by stirring or shaking Bioresource Technology, 102(10), pp. 6295-6298. Elsevier 10.1016/j.biortech.2011.02.016

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Bovine serum albumin (BSA) was applied as a model non-catalytic protein to enzymatic hydrolysis of Avicel and dilute acid pretreated corn stover at different reaction conditions to improve the understanding of its ability to enhance cellulose hydrolysis. Addition of BSA improved the 72 h hydrolysis yields in shake flasks by up to 26% for both substrates by reducing de-activation of the exoglucanases and by facilitating reductions in particle size and crystallinity during a magnetically stirred pre-incubation step. The enzyme stabilizing effect of BSA addition was most striking for batch hydrolysis in a stirred tank reactor, with glucose yields increasing by 76% after 72 h for Avicel and by 40% after 145 h for corn stover. Application of BSA to continuous hydrolysis for a mean residence time of 24h gave 33% and 40% higher glucose yields for corn stover and Avicel compared to the controls.

Item Type:	Journal Article (Original Article)
Division/Institute:	School of Agricultural, Forest and Food Sciences HAFL > Resource-efficient agricultural production systems School of Agricultural, Forest and Food Sciences HAFL > Agriculture
Name:	Brethauer Studer, Simone0000-0001-7755-7593; Studer, Michael Hans-Peter0000-0003-1083-0967; Yang, Bin and Wyman, Charles E.
Subjects:	Q Science > Q Science (General)
ISSN:	09608524
Publisher:	Elsevier
Language:	English
Submitter:	Michael Hans-Peter Studer
Date Deposited:	03 Mar 2020 11:04
Last Modified:	18 Dec 2020 13:29
Publisher DOI:	10.1016/j.biortech.2011.02.016
Uncontrolled Keywords:	BSA, Enzymatic hydrolysis, Continuous hydrolysis, Enzyme stability, Cellulose
ARBOR DOI:	10.24451/arbor.10126
URI:	https://arbor.bfh.ch/id/eprint/10126