A Multispecies Fungal Biofilm Approach to Enhance the Celluloyltic Efficiency of Membrane Reactors for Consolidated Bioprocessing of Plant Biomass

Xiros, Charilaos; Studer, Michael Hans-Peter (2017). A Multispecies Fungal Biofilm Approach to Enhance the Celluloyltic Efficiency of Membrane Reactors for Consolidated Bioprocessing of Plant Biomass Frontiers in Microbiology, 8 Frontiers Research Foundation 10.3389/fmicb.2017.01930

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The constraints and advantages in cellulolytic enzymes production by fungal biofilms for a consolidated bioconversion process were investigated during this study. The biofilm cultivations were carried out in reactors designed for consolidated bioprocessing Multispecies Biofilm Membrane reactors, (MBM) where an aerobic fungal biofilm produces the lignocellulolytic enzymes while a fermenting microorganism forms the fermentation product at anaerobic conditions. It was shown that although mycelial growth was limited in the MBM reactors compared to submerged cultivations, the secretion of cellulolytic enzymes per cell dry weight was higher. When Trichoderma reesei was used as the sole enzyme producer, cellobiose accumulated in the liquid medium as the result of the deficiency of β-glucosidase in the fungal secretome. To enhance β-glucosidase activity, T. reesei was co-cultivated with A. phoenicis which is a β-glucosidase overproducer. The two fungi formed a multispecies biofilm which produced a balanced cellulolytic cocktail for the saccharification of plant biomass. The mixed biofilm reached a 2.5 fold increase in β-glucosidase production, compared to the single T. reesei biofilm. The enzymatic systems of single and mixed biofilms were evaluated regarding their efficiency on cellulosic substrates degradation. Washed solids from steam pretreated beechwood, as well as microcrystalline cellulose were used as the substrates. The enzymatic system of the multispecies biofilm released four times more glucose than the enzymatic system of T. reesei alone from both substrates and hydrolyzed 78 and 60% of the cellulose content of washed solids from beechwood and microcrystalline cellulose, respectively.

Item Type:

Journal Article (Original Article)

Division/Institute:

School of Agricultural, Forest and Food Sciences > Abteilung Agronomie

Name:

Xiros, Charilaos and Studer, Michael Hans-Peter

Subjects:

Q Science > QR Microbiology

ISSN:

1664-302X

Publisher:

Frontiers Research Foundation

Funders:

[UNSPECIFIED] Swiss National Science Foundation SNSF

Language:

English

Submitter:

Michael Hans-Peter Studer

Date Deposited:

03 Mar 2020 11:07

Last Modified:

28 Apr 2020 11:33

Publisher DOI:

10.3389/fmicb.2017.01930

Uncontrolled Keywords:

multispecies biofilm membrane reactors, Trichoderma reesei, Aspergillus phoenicis, lignocellulose, cellulases, β-glucosidase, fungal biofilms

ARBOR DOI:

10.24451/arbor.10119

URI:

https://arbor.bfh.ch/id/eprint/10119

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